nmo is co-expressed with the RDGN in the second instar eye disc. Expression of the nmo-lacZ enhancer trap during second instar eye disc development [63–72 hr after egg laying (AEL)], detected with anti-β-gal antibody. (A) nmo-lacZ is expressed in all cells. (B–G) nmo-lacZ (green, B and E) coincides with Eya (red, C and F) in the posterior eye disc in mid (B–D) and late (E–G) second instar. (H) Schematic summarizing nmo's co-expression with the eye-specification genes. Early: nmo and Ey are co-expressed in the posterior eye field. Mid: nmo is co-expressed with Ey in anterior cells of the eye field and with Ey and Eya in posterior cells. Late: same is in mid, except at the posterior margin where nmo is co-expressed with Ey, Eya, and Dac.