Relative fitness components of the following clones: ancestor (uspA/B −IS150), evolved clone AncB/FTG uspA/B +IS150, and constructed clone AncB/FTG uspA/B −IS150. All three genotypes competed against the ancestor with the opposite arabinose marker. Each relative fitness component is as follows: (A) overall FTG fitness, (B) FT survival, (C) growth after FT, and (D) growth after stationary phase. Error bars are 95% confidence intervals of 12 replicate assays.