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. Author manuscript; available in PMC: 2008 Sep 13.
Published in final edited form as: Mol Cell. 2008 Jul 25;31(2):294–301. doi: 10.1016/j.molcel.2008.06.016

Figure 4. Highly Efficient Gene Targeting of Endogenous Human Loci by OPEN ZFNs.

Figure 4

(A) OPEN VEGF-A ZFNs and previously described IL2RγZFNs induce efficient gene targeting at endogenous genes in human K562 cells. Top part shows representative gel images from limited-cycle PCR/restriction digest assays and bottom part shows gene targeting frequency means (colored bars) and standard errors (error bars) from multiple experiments.

(B) Gene targeting efficiencies of OPEN VEGF-A ZFNs assessed nine days post-transfection by limited-cycle PCR/restriction digest and Southern blot assays.

(C) Vinblastine enhances gene targeting by OPEN VEGF-A and four-finger IL2RγZFNs. Assays performed four days post-transfection. Data presented as in (A).

(D) Toxicities of OPEN VEGF-A and four-finger IL2RγZFNs in human K562 cells. Means of GFP (green bars) and gene targeting ratios (purple bars) are shown. Error bars represent standard deviations. Single and double asterisks indicate p values <0.05 or <0.01, respectively.

(E) Gene targeting efficiencies of OPEN VEGF-A and four-finger IL2RγZFNs in toxicity experiments of (D). Means and standard deviations (error bars) are shown of PCR-based assays performed four days post-transfection.