. Author manuscript; available in PMC: 2009 Jan 18.

Published in final edited form as: ACS Chem Biol. 2008 Jan 5;3(1):30–37. doi: 10.1021/cb7002225

Table 1.

Protective groups and general conditions for solid-phase oligoribonucleotide synthesis

5′▵	2	3′●	support	5′ deprotection	activator, coupling time, efficiency	oxidation	2′ deprotection	ref
TBDMS	DMT	CNEt	CPG	TCA	tetrazole, 6–12 min, >95%	I₂^a	i) fluoride (TBAF)	24
Fpmp	DMT	CNEt	CPG	TCA	tetrazole, 3–15 min, >98%	I₂^a	ii) acid (NaOAc/pH 3.25)	20
Nbn	DMT	CNEt	CPG	TCA	tetrazole, 5–15 min, ~95%	I₂^a	iii) hν8 nm)	8
DTM	DMT	CNEt	CPG	TCA	ETT, 150 sec, >98%	I₂^a	iv) reduction (DTT or TCEP)	21
allyl	DMT	allyl	ref. 53	TCA	tetrazole, 12 min, >93%	t-C₄H₉OOH	i) metal Pd₂(dba)₃	22
TOM	DMT	CNEt	CPG	DCA	BTT, 2.5 min, >99%	I₂^a	i) fluoride(TBAF)	26
ACE	BzH	Me	P	HF/TEA	ETT, 90 sec, >99%	t-C₄H₉OOH	ii) acid (HOAc/TEMED/pH 3.8)	27

I₂/H₂O/THF/Pyr