Table 1.
Strains and plasmids used in this study
| Strain | Genotype | Source |
|---|---|---|
| MY2787 | MATα ura3-52 leu2-Δ1 lys2-Δ202 | This laboratorya |
| MY2792 | MATα ura3-52 leu2-Δ1 his3-Δ200 | This laboratory |
| MY3371 | MATa ura3-52 leu2-Δ1 | This laboratory |
| MY3608 | MATa leu2 ura3-35 spa2-964 | This laboratory |
| MY3773 | MATα spa2-964 lys2-Δ202 ura3-52 | This laboratory |
| MY3776 | MATa leu2 ura3-52 spa2-Δ3::URA3 | This laboratory |
| MY3785 | MATα rvs161-1811 leu2 lys2-Δ202 ura3-52 | This laboratory |
| MY3905 | MATα rvs161-Δ1::LEU2 ura3-52 leu2-Δ1 his3-Δ200 | This laboratory |
| MY3909 | MATa rvs161-Δ1::LEU2 ura3-52 his3-Δ200 leu2-Δ1 trp1-Δ63 | This laboratory |
| MY4158 | MATa ura3-52 leu2 fus2-410 | This laboratory |
| MY4160 | MATa fus1-Δ1 fus2-Δ ura3-52 lys2-Δ202 | This laboratary |
| MY4161 | MATa trp1-Δ1 ura3-52 fus1-Δ1 | This laboratory |
| MY4164 | MATα trp1-Δ1 ura3-52 fus1-Δ1 | This laboratory |
| MY4178 | MATα ura3-52 leu2 lys2-Δ202 fus2-410 | This laboratory |
| MY4477 | MATa ura3-52 leu2 his3-Δ200 rvs161-1811 | This laboratory |
| MY4495 | MATα ura3-52 leu2 lys2-801 his3-Δ200 rvs161-Δ1::LEU2 | This laboratory |
| MY4742 | MATa fus2-Δ3 his4-Δ34 leu2-3 leu2-112 ura3-52 rvs161-Δ2::URA3 | This laboratory |
| MY4744 | MATα fus2-Δ3 his4-Δ34 trp1-Δ1 ura3-52 canr rvs161-Δ2::URA3 | This laboratory |
| MY4746 | MATa leu2 ura3-52 spa2-964 rvs161-Δ2::URA3 | This laboratory |
| MY4748 | MATα lys2-Δ202 ura3-52 spa2-964 rvs161-Δ2::URA3 | This laboratory |
| MY4801 | MATa fus2-Δ3 rvs161-Δ2::URA3 his4-Δ34 leu2-3 leu2-112 ura3-52 | This laboratory |
| MY4802 | MATα fus2-Δ3 rvs161-Δ2::URA3 his4-Δ34 trp1-Δ1 ura3-52 canr | This laboratory |
| MY4813 | MATa spa2-964 fus2-Δ::URA3 ura3-52 leu2 | This laboratory |
| MY4815 | MATα spa2-964 fus2-Δ::URA3 ura3-52 lys2-Δ202 | This laboratory |
| MY4817 | MATa spa2-964 fus1-Δ1 ura3-52 leu2 | This laboratory |
| MY4819 | MATα spa2-964 fus1-Δ1 ura3-52 lys2-Δ202 | This laboratory |
| MY4843 | MATα trp1-Δ1 ura3-52 fus1-Δ1 fus2-Δ3 p0 | This laboratory |
| MY4859 | MATa ura3-52 his3-Δ200 leu2-Δ1 trp1-Δ63 rvs161-Δ::LEU2 fus2-Δ::URA3 | This laboratory |
| MY4905 | MATa ura3-52 his3-Δ200 leu2-Δ1 trp1-Δ63 rvs1671::LEU2 fus1-Δ1 | This laboratory |
| MY4907 | MATα leu2 ura3-52 his3-Δ200 lys2-801 rvs161::LEU2 fus1-Δ1 | This laboratory |
| MY5040 | MATα leu2 ura3-52 his3-Δ200 lys2-801 rvs161::LEU2 fus2-Δ::URA3 | This laboratory |
| MY5062 | MATa spa2-964 rvs161-Δ2::ura3 leu2 ura3-52 | This laboratory |
| MY5067 | MATa spa2-964 fus2-Δ::ura3 leu2 ura3-52 | This laboratory |
| MY5224 | MATa ura3-52 his3-Δ200 leu2-Δ1 trp1-Δ63 rvs161-P203Q | This laboratory |
| MY5359 | MATα ura3-52 leu2-Δ1 rvs161-P203Q | This laboratory |
| MS2073 | MAT::LEU2 leu2-3 leu2-112 ura3-52 his3::TRP1 trp1-Δ1 | This laboratory |
| MS2104 | MAT::LEU2 leu2-3 leu2-112 ura3-52 his3::TRP1 trp1-Δ1 [MATα URA3] | This laboratory |
| MS2679 | MAT::LEU2 fus6-964 leu2-3 leu2-112 ura3-52 his3::TRP1 trp1-Δ1 [MATα URA3] | This laboratory |
| MS2680 | MAT::LEU2 fus6-964 leu2-3 leu2-112 ura3-52 his3::TRP1 trp1-Δ1 | This laboratory |
| MS2749 | MAT::LEU2 fus2-410 leu2-3 leu2-112 ura3-52 his3::TRP1 trp1-Δ1 [MATα URA3] | This laboratory |
| Y601 | MATα spa2-Δ3::URA3 ura3-52 his3-δ200 trp1-901 ade2-101 | M. Snyder, Yale University |
| JY424 | MATa fus2-Δ3 his4-Δ34 leu2-3 leu2-112 ura3-52 | G. Fink, Whitehead Institute |
| JY427 | MATa fus1-Δ1 leu2-3 leu2-112 ura3-52 | G. Fink, Whitehead Institute |
| JY428 | MATα fus2-Δ3 his4-Δ34 trp1-Δ1 ura3-52 canr | G. Fink, Whitehead Institute |
| JY429 | MATα trp1-Δ1 ura3-52 fus1-Δ1 fus2-Δ3 | G. Fink, Whitehead Institute |
| Plasmid | Yeast markers | Source |
| pMR3093 | SPA2 CEN ARS URA3 | This laboratory |
| pMR3234 | RVS161 CEN ARA URA3 | This laboratory |
| pMR3397 | RVS161 2μ URA3 | This laboratory |
| pMR3245 | rvs161-Δ1::LEU2 | This laboratory |
| pMR3261 | rvs161-Δ2::URA3 | This laboratory |
| pSB245 | FUS1 CEN ARS URA3 | G. Fink, Whitehead Institute |
| pSB257 | FUS2 2μ URA3 | G. Fink, Whitehead Institute |
| pSB265 | FUS2 CEN ARS URA3 | G. Fink, Whitehead Institute |
| pSB273 | FUS1 2μ URA3 | G. Fink, Whitehead Institute |
| pSB267 | fus2-Δ::URA3 | G. Fink, Whitehead Institute |
| pSB281 | fus1-Δ1 URA3 | G. Fink, Whitehead Institute |
| p182 | SPA2 2μ URA3 | M. Snyder, Yale University |
| pTS395 | PGAL-GFP CEN ARS URA3 | T. Stearns, Stanford University |
| pRS426 | 2μ URA3 | P. Hieter, Johns Hopkins |
| pRS424 | 2μ TRP1 | P. Hieter, Johns Hopkins |
a
The MS strains from the Rose Laboratory are congenic with S288C (hap2 mal kss1 gal2). The MY strains are also congenic with S288C, except that they are GAL2.