Table 2.

Vesicle localization and accumulation at the zone of cell fusion in prezygotes

Matinga	% with vesiclesb	Vesicles per zonec	% aligned vesiclesd	% with plaquese	% with invaginationf
1. WT	71	13	100	0	0
2. fus1	30	6	47	0	0
3. spa2	60	19	4	0	0
4. fus2	33	15	100	20	6
5. rvs161Δ	50	29	98	15	13
6. rvs161-P203Q	80	12	100	10	25
7. fus1 fus2	12	7	40	0	0
8. fus1 rvs161	12	9	37	0	20
9. fus1 spa2	30	10	0	0	0
10. fus2 spa2	67	8	0	0	0
11. spa2 rvs161	80	9	0	0	7
12. fus2 rvs161	45	25	100	27	21

^a The data are from the following matings: 1. WT (MY3371 × MY2787); 2. fus1 (MY4161 × MY4164); 3. spa2 (MY3608 × MY3773); 4. fus2 (JY424 × JY428); 5. rvs161 (MY3909 × MY4495); 6. rvs161-P203Q (MY5224 × MY5359); 7. fus1 fus2 (MY4160 × JY429); 8. fus1 rvs161 (MY4905 × MY4907); 9. fus1 spa2 (MY4817 × MY4819); 10. fus2 spa2 (MY4813 × MY4815); 11. spa2 rvs161 (MY4746 × MY4748); and 12. fus2 rvs161 (MY4859 × MY4802). 

^b The number of prezygotes with vesicles that could be scored divided by the total number of zygotes is represented in the % with vesicles column. 

^c The number of vesicles at the cell fusion zone per 70 to 90-nm section is shown in the vesicles per zone column. 

^d The prezygotes were scored as having aligned vesicles (% aligned) if the vesicles were clustered in ∼0.5-μm-diameter regions directly across the cell fusion zone. 

^e The percentages of prezygotes with electron-dense regions on one or both sides of the cell fusion zone (Figure 6) were scored as containing plaques (% with plaques). 

^f The percentage of prezygotes with one or more invagination (Figure 6) is indicated.