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. Author manuscript; available in PMC: 2008 Sep 15.
Published in final edited form as: J Am Chem Soc. 2007 Jun 19;129(27):8400–8401. doi: 10.1021/ja070238o

Figure 5.

Figure 5

Labeling of live cells bearing two bioorthogonal chemical reporters. Jurkat cells were incubated with combinations of Ac4ManLev (50 μM), Ac4GalNAz (150 μM), and no sugar for 2 d, followed by labeling with both 1 and biotin hydrazide at pH 6.5 and, subsequently, with FITC-avidin. The fluorescence of each sample was measured by flow cytometry. x-axis: FITC fluorescence; y-axis: Cy5.5 fluorescence. (A) – Ac4ManLev, + Ac4GalNAz. (B) + Ac4ManLev, + Ac4GalNAz. (C) – Ac4ManLev, – Ac4GalNAz. (D) + Ac4ManLev, – Ac4GalNAz.