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Chromatogram of an oxidative folding mixture of unONC (containing 10% cyclized ONC) at 4 hours at pH 8, 25 mM DTTox. All free thiols in each sample were blocked with AEMTS, and the samples were desalted prior to analysis using cation-exchange HPLC. After desalting, the blocked protein was incubated without QC (A) and with QC (B) at pH 7.2, room temperature. The unstructured species are indicated with braces.
