| 1 bottle Ames solution (Sigma, 8.8g) |
| 1.9 gm sodium bicarbonate |
| 10 ml 1% pen/strep/L-Glutamine (1:100) (Gibco/Invitrogen) |
| 990 ml distilled water to 1L |
| ------------------------------- |
| 1 Liter |
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