Figure 2. RAD51 depleted cells form radials.
Metaphase spreads of normal GM639 cells treated with A) negative control scrambled siRNA and no MMC B) RAD51 siRNA and no MMC showing spontaneous radial formation (arrows), and C) RAD51 siRNA and 40 ng/ml of MMC. D) Depletion of RAD51 from normal GM639 cells caused increased spontaneous radials (*), with further increase following MMC treatment.