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. Author manuscript; available in PMC: 2008 Sep 15.
Published in final edited form as: Biochemistry. 2007 Jan 9;46(1):271–278. doi: 10.1021/bi061858p

FIGURE 3.

FIGURE 3

Ime2 phosphorylates a peptide containing the Sum1-phosphorylation consensus. A peptide RKERPSTAN (■), which contains the sequence 301-308 of Sum1, or the same peptide lacking the phosphoacceptor, RKERPSAAN (□), were incubated with Ime2 prepared from sporulating cells. (A) Time course of phosphorylation using 10 μM peptide (n=3). (B) Michaelis-Menten plot of peptide phosphorylation (n=3).