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. Author manuscript; available in PMC: 2008 Sep 15.
Published in final edited form as: Biochemistry. 2007 Jan 9;46(1):271–278. doi: 10.1021/bi061858p

TABLE 1.

Yeast strains used in this study.

Strain Genotype Source
KSY187 MATa/MATα ura3/ura3 leu2::hisG/leu2::hisG
trp1ΔFA::hisG/trp1ΔFA::hisG lys2/lys2
ho::hisG/ho::hisG IME2-myc::TRP1/IME2-myc::TRP1
(ref. 22)
KSY162 KSY187 + ime2-K97R-myc/ime2-K97R-myc (ref. 22)
MSY4 MATa/MATα ura3/ura3 leu2::hisG/leu2::hisG
trp1ΔFA::hisG/trp1ΔFA::hisG lys2/lys2 arg4-
NspI/ARG4 ho::hisG/ho::hisG RME1/rme1::LEU2
gip1::HIS3/ gip1::HIS3
A. Neiman
(ref. 29)
MSY3 MATa/MATα ura3-1/ura3-1 his3-11,15/his3-11,15
leu2-3,115/leu2-3,115 trp1-1/trp1-1 met15-Δ/met15-Δ
sgs1::HIS3/sgs1::HIS3 can1-100/can1-100 ade2-
ΔNdeI/ade2-ΔAatII
R. Rothstein
(ref. 26)
LNY150 MATa/MATa ura3/ura3 leu2::hisG/leu2::hisG
trp1::hisG/trp1::hisG lys2/lys2 his4-N/his4-G
ho::LYS2/ho::LYS2
This study
MMY35 LNY150 + SUM1-T306A/SUM1-T306A This study
EWY88 MATa/MATa ura3/ura3 leu2::hisG/leu2::hisG
lys2/lys2 ho::LYS2/ho::LYS2 dmc1::ARG4/dmc1::ARG4
This study
(ref. 13)
MMY36 EWY88 + SUM1-T306A/SUM1-T306A This study