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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Alcohol. 2008 Jun 24;42(6):499–508. doi: 10.1016/j.alcohol.2008.04.009

Figure 2.

Figure 2

Maximum ethanol potentiation (see methods) from pooled [3H]DA uptake data in SKN-SH and HEK-293 cells expressing wild-type or G130T mutant DAT. Control cells, expressing wild-type or G130T DAT, were incubated in growth media with 100 nM [3H]DA without ethanol. Ethanol significantly potentiated transporter-mediated [3H]DA uptake compare to untreated controls in SK-N-SH cells expressing wild-type DAT or G130T DAT 51% AND 29%, respectively. Potentiation was significantly reduced in SK-N-SH cells expressing the G130T mutant compared to wild-type DAT expressing cells. Ethanol potentiated transporter-mediated [3H]DA uptake in HEK-293 cells expressing wild-type DAT or G130 DAT 55% and 28%, respectively. Potentiation was significantly reduced in HEK-293 cells expressing the G130T mutant compared to wild-type DAT expressing cells. Data are expressed as mean ± SEM of maximal uptake values from three experiments. *p < 0.05 compared with untreated control. **p<0.05 compared with wild-type DAT (Student's t test).