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. Author manuscript; available in PMC: 2008 Sep 15.
Published in final edited form as: J Cell Sci. 2008 Apr 29;121(Pt 10):1661–1670. doi: 10.1242/jcs.020149

Fig. 10.

Fig. 10

PAI-1 does not disrupt matrix adhesions. Cells were incubated overnight onto coverslips in complete media and then incubated for 3 hours in DMEM in the presence or absence of PAI-1(20 nM). Cells were subsequently fixed, and permeabilized. A - Fibronectin and tensin were visualized by indirect immunofluorescence in non-treated cells (panels a and b) and PAI-1 treated cells (panels d and e). Panels c and e show the merged pictures. Yellow staining represents area of colocalization of tensin and fibronectin. Scale Bar, 20 μm. B – Paxillin and tensin were visualized by direct and indirect immunofluorescence in non-treated cells (panels a and b) and PAI-1 treated cells (panels d and e). Panels c and f shows merged pictures. Scale Bar, 20 μm.