Fig. 11.

β5 knockdown inhibits PAI-1 stimulation of matrix assembly. A - Lysates from MG-63 cells treated with either control siRNA or β5 siRNA were analyzed for β5 integrin and actin by Western blot. B, C - MG-63 cells treated with either control or β5 siRNA were incubated with ¹²⁵I-fibronectin for 6 hours in the presence of 20 nM PAI. Cells were extracted with 1% DOC and ¹²⁵I-fibronectin incorporated into detergent insoluble matrix was recovered by centrifugation and measured by gamma scintillation. Experiments are representative of separate experiments performed in triplicate. Data are the mean ± S.E. of one experiment performed in triplicate. t test, p<0.05 (n=3). All values were normalized to protein content. No significant difference in protein content was observed between control siRNA and β5 siRNA treated cells.