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. Author manuscript; available in PMC: 2008 Sep 15.
Published in final edited form as: J Cell Sci. 2008 Apr 29;121(Pt 10):1661–1670. doi: 10.1242/jcs.020149

Fig. 7.

Fig. 7

PAI-1 disrupts actin stress fibers. MG-63 cell monolayers were plated overnight onto coverslips in complete media and then incubated for 3 hours with 20 nM of PAI-1 or PAI-1 mutants (PAI-1R and Q123K). Cells were subsequently fixed, permeabilized and stained for 1 hour with the clone 15F11 antibody for β5 integrin. Cells were then washed and labeled for an additional hour with the AlexaFluor488 derivatized anti-mouse secondary antibody and the AlexaFluor594-phalloidin. Actin and β5 integrin were visualized by indirect immunofluorescence. Scale Bar, 20 μm.