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Effects of PD98059, LY294002, and rapamycin on agar colony formation by CEF expressing v-Src. CEF infected with RCAN(A)-BH-v-Src at 0.1 multiplicity of infection were trypsinized and resuspended in agar suspension containing no inhibitor, PD98059, LY294002, rapamycin, PD98059 and LY294002, or PD98059 and rapamycin. Suspension cultures, which were fed every 3 d with agar overlay containing inhibitors, were incubated for 10–12 d at 41°C.
