Table 5.
Expression of luciferase activity after infection or transduction with MuLV-Luc viral vector
| Cell line | Virus infectiona
|
DNA transfectionb
|
||||
|---|---|---|---|---|---|---|
| Prep 1 | Prep 2 | Prep 3 | 20 ng | 200 ng | 2 μg | |
| RC-2 | 11,043 | 12,698 | 5,188 | 1,609 | 11,586 | 111,296 |
| R3-2 | 400 | 522 | 191 | 7,410 | 57,421 | 498,101 |
| R4-7 | 428 | 323 | 146 | 4,135 | 19,626 | 178,160 |
a
Cells were infected with different preparations of ecotropic MuLV-Luc virus, and lysates were assayed for luciferase activity. The values are normalized to input protein.
b
Cells were transfected with the indicated amounts of MuLV-Luc DNA, and lysates were assayed for luciferase activity. The values are normalized to input protein.