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. 1998 Jun;9(6):1411–1424. doi: 10.1091/mbc.9.6.1411

Figure 6.

Figure 6

Macrophage-derived TNF-α contributes to fibroblast collagenase-3 induction. (A) Murine monocytic J774 cells were cultured for 24 h alone (lane 1) or in the presence of 50 ng/ml phorbol-myristate acetate (lane 2) or 4 mg/ml zymosan (lane 3) and harvested for Northern blot analysis of TNF-α mRNA. (B) Murine monocytic J774 cells were allowed to condition medium for 24 h in the presence of zymosan (4 mg/ml). Primary cultures of ALFs were untreated (lane 1) or were treated with 50% zymosan-treated J774-conditioned medium (CM) alone (lane 2), with 20 ng/ml recombinant murine TNF-α alone (lane 3), or with 50% zymosan-treated J774-CM pretreated with 10 μg/ml TNF-α-neutralizing antibody (lane 4) or 10 μg/ml IL-1β-neutralizing antibody (lane 5). After 48 h, cells were harvested for Northern blot analysis of collagenase-3 (C’ase) and glyceraldehyde-3-phosphate dehydrogenase (GAP) mRNAs.