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. 1999 Jun;10(6):1719–1732. doi: 10.1091/mbc.10.6.1719

Figure 3.

Figure 3

The CPY sorting defect in vti1-1 and vti1-2 cells is suppressed by overproduction of Pep12p but not by Vam3p. Cells were grown at 24°C, shifted to 36°C (A and B) or 31°C (C) for 15 min, labeled for 10 min at that temperature, and chased for 30 min. CPY was immunoprecipitated from cellular extracts (I) and extracellular fractions (E) and analyzed by SDS-PAGE. (A) In vti1 mutant cells the Golgi-modified p2CPY accumulated within the cells (I) and was secreted (E). Overproduction of Pep12p led to the production of mature CPY (mCPY) in vti1-1 cells (A) and in vti1-2 cells (C) at a semipermissive temperature (31°C). At 36°C the CPY sorting defect could not be suppressed in vti1-2 cells (B). Overproduction of Vam3p had no effect on CPY sorting in either vti1-1 or vti1-2 cells.