Skip to main content
. 1999 Jun;10(6):1719–1732. doi: 10.1091/mbc.10.6.1719

Figure 8.

Figure 8

Nyv1p is not required for transport of CPY, ALP, or API into the vacuole. Transport was analyzed in wild-type cells, nyv1Δ cells, vti1-2 cells, vti1-2 nyv1Δ double mutant cells, and vti1-2 cells overexpressing NYV1 from a 2μ plasmid. (A) CPY traffic was followed by pulse–chase immunoprecipitations at 31°C. CPY was transported to the vacuole and processed in nyv1Δ cells (mCPY). CPY traffic at the semipermissive temperature was affected to a similar degree in vti1-2, vti1-2 nyv1Δ double mutant and vti1-2 cells overexpressing NYV1. (B) ALP traffic was analyzed at 31°C by pulse–chase immunoprecipitations. ALP was delivered to the vacuole with normal kinetics in nyv1Δ cells. Deletion or overexpression of NYV1 did not change ALP traffic in a vti1-2 background. (C) API traffic was studied by pulse–chase immunoprecipitations at 36°C. API transport was unaffected in nyv1Δ cells. Deletion or overexpression of NYV1 did not influence API processing in a vti1-2 background. These data indicate that NYV1 is not required for CPY, ALP, or API traffic, and that VTI1 and NYV1 do not interact genetically.