Figure 3.
Colocalization of α8tβ1-AP binding sites, OPN, and the integrin α8 subunit in the developing kidney. Paraffin sections (A and C) and frozen sections (B) of an E16.5 kidney were incubated with α8tβ1-AP (A), anti-OPN peptide antiserum (B), and affinity-purified anti-mouse integrin α8 subunit IgG (C). The panels show a cross-section through the ureter epithelium, and the arrows point to the border between the epithelium and surrounding mesenchyme. Note that the section is at a level below the tips of the ureter; thererfore, no condensing mesenchymal cells are visible. Bar, 50 μm.