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Cell-spreading assay. In the presence of 1 mM MnCl2, K562 cells (A, C, E, and G) and KA8 cells (B, D, F, and H) were plated onto different substrata: (A and B) BSA; (C and D) FN (coated at 5 μg/ml); (E and F) purified native OPN (5 μg/ml); and (G and H) recombinant GST-OPN (5 μg/ml). Note that both cell types spread on FN, but only KA8 cells spread on native OPN and recombinant GST-OPN. Bar, 100 μm.
