Figure 4.
Demonstration of selective release of adherent cells under electrochemical control. An optical micrograph showed that Swiss 3T3 fibroblast cells adhered to the circular regions that presented RGD ligands (a). After treatment with an electrical potential of 650 mV, cells were efficiently released only from regions presenting electroactive O-silyl hydroquinone (b), while an electrical potential of −650 mV resulted in release of cells only from regions presenting electroactive quinone ester (c). Subsequent application of an electrical potential of −650 mV to the monolayer of panel b and of 650 mV to the monolayer of panel c caused additional release of cells from regions that present remaining electroactive groups (d). Note that the image shown in panel c was taken from a separate experiment.