Larval growth arrest phenotype and survival of ppan mutant larvae. (A) Heterozygous and homozygous ppan larvae at 24, 48, 72, 96, 120, 144, and 168 h AED. (B) Larval growth curve for normally fed wild-type (black squares), sucrose-fed wild-type (open triangles), and normally fed homozygous ppan (black circles) larvae. Groups of ≥20 larvae were weighed at various times after hatching, and an average weight per larva was determined. (C) Survival of hatched ppan larvae Mutant ppan larvae were counted for several days after hatching.