Figure 5.

The ppan gene complements ssf1 and ssf2 function. The yeast strain H50[hyphem]16C is deficient for ssf1 and ssf2 but has the ssf1 gene added back on a plasmid under the control of a galactose promoter. Thus these cells can only grow in media containing galactose. These cells were transformed to ura⁻ galactose plates with either the empty pAB23BXN plasmid or the same plasmid with ppan subcloned into it under the transcriptional control of the strong constitutive GAPDH promoter. The streaked colonies from both the control and ppan transformations were replated onto ura⁻ dextrose plates. The ppan expressing colonies (pAB23BXN-ppan) were able to grow, whereas the control colonies (pAB23BXN) were not.