Ectopic overexpression of ppan causes cell death and disrupts wing development. Results of ppan overexpression in the posterior compartment of the imaginal wing disc using the engrailed GAL4 driver: (A) en-GAL4; (B) en-GAL4, UAS-ppan. Results of ppan overexpression in the imaginal wing disc using the ms 1096-GAL4 driver, which expresses GAL4 preferentially in the dorsal portion of the disc: (C) ms 1096 GAL4, UAS-ppan; (D) ms 1096 GAL4, UAS-ppan, UAS-P35. (E) Closeup of irregular and duplicated wing trichomes in ms 1096-GAL4, UAS-ppan/+ flies. Visualization of cell death in imaginal discs using acridine orange: (F) wild-type; (G) en-GAL4, UAS-ppan. Arrows indicate abnormal cell death in the posterior wing compartment.