Table 1.

Mosaic analysis: ppan mutant cells have a growth defect

HS (h AED)	Genotype	ppan clone survival (L3 discs)	Morphology/clone survival (adults)
96	FRT ppan/FRT	+++	Normal/many small clones
72	FRT ppan/FRT	+	Normal/many small clones
48	FRT ppan/FRT	−	Normal/no surviving clones
36	FRT ppan/FRT	−	Normal/no surviving clones
144	FRT ppan/FRT Minute	+++	Rough eyes, wing and cuticle defects/ND
120	FRT ppan/FRT Minute	+++	Rough eyes, wing and cuticle defects/ND
96	FRT ppan/FRT Minute	++ (>30 cells)	Pupal lethal/ND
72	FRT ppan/FRT Minute	++ (>50 cells)	Pupal lethal/ND

Data summary for studies of ppan clones allowed to grow for various times after heat shock (HS) induction of flp recombinase (see MATERIALS AND METHODS). For analysis in L3 discs, larvae were fixed at 120 h AED (wild-type background) and at 168 h AED (Minute background). Mutant clones in discs were identified using the πmyc marker and in adult eyes by pigmentation derived from the w⁺ marker in P[w⁺ ppan^6B6]. +++, mutant clones equivalent in size to wild-type sister clones (“twin spots”); ++ and +, mutant clones smaller than twin spots; −, no detectable mutant clones; ND, clone survival was not determined.