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. 2008 Jul 10;17(19):3021–3029. doi: 10.1093/hmg/ddn200

Figure 4.

Figure 4.

The H19 DMD is required to prevent interactions between the maternal endodermal enhancers and the Igf2 promoters. The 3C analysis was carried out on samples from a H19Δ3.8 kb-5′H19 × B6(CAST7) cross. (A) Top, schematic of the H19Δ3.8 kb-5′H19 allele (see Fig. 2 for details), with 3.8 kb upstream of the H19 promoter removed. Bottom, representative gel image of 3C PCR products: D, digested with NlaIII, which distinguishes paternal (P) from maternal (M) alleles; 3C, non-digested PCR product; -L, no ligase control; +, positive control. When the H19Δ3.8 kb-5′H19 allele is inherited maternally, the endodermal enhancers on the maternal chromosome interact with the whole H19 region. (B) Top, schematic of the Igf2 locus (see Fig. 3 for details). Bottom, representative gel image of 3C PCR products digested with HaeIII, which distinguishes paternal (P) from maternal (M) alleles.