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. 2008 Jul 10;17(19):3021–3029. doi: 10.1093/hmg/ddn200

Figure 5.

Figure 5.

The CTCF sites are necessary for the enhancer-blocking activity of the DMD. The 3C analysis was carried out on samples from a H19DMD-ΔR × B6(CAST7) cross. (A) Top, schematic of the H19DMD-ΔR allele, in which the CTCF sites in the DMD have been deleted (see Fig. 2 for details). Bottom, representative gel image of 3C PCR products: D, digested with NlaIII, which distinguishes paternal (P) from maternal (M) alleles; 3C, non-digested PCR product; -L, no ligase control; +, positive control. (B) Top, schematic of the Igf2 locus (see Fig. 3 for details). Bottom, representative gel image of 3C PCR products digested with HaeIII, which distinguishes paternal (P) from maternal (M) alleles.