Table 4. Real-time PCR results.
|
Transforming growth factor beta 2 | ||||
|
Sample |
Green Signal |
Red Signal |
Array |
Real-Time |
| 1A |
73.9** |
45.3 |
-1.38 |
0.77 |
| 2A |
21.2 |
9* |
-2.35 |
0.26 |
| 4A |
850.6** |
83.2 |
-10.20 |
0.04 |
| 7A |
263.9** |
43 |
-96.06 |
0.15 |
| Mean |
-5.00 |
0.31 |
||
|
Matrix metalloproteinase 9 | ||||
|
Sample |
Green Signal |
Red Signal |
Array |
Real-Time |
| 1A |
29.7 |
106.8 |
3.60 |
727.2 |
| 2A |
18.4 |
158.9 |
8.64 |
1928.9 |
| 4A |
26.2 |
69.8 |
2.67 |
574.4 |
| 7A |
30.9 |
110.8 |
3.59 |
1020.4 |
| Mean |
4.62 |
1062.7 |
||
|
Interleukin-1 beta | ||||
|
Sample |
Green Signal |
Red Signal |
Array |
Real-Time |
| 1A |
14 |
254.8 |
18.25 |
86.03 |
| 2A |
4.3* |
50.4 |
11.69 |
258.92 |
| 4A |
2* |
11.1* |
5.43 |
67.75 |
| 7A |
17.2 |
306.1 |
17.82 |
146.83 |
| Mean |
13.3 |
139.9 |
||
|
Fibronectin | ||||
|
Sample |
Green Signal |
Red Signal |
Array |
Real-Time |
| 1A |
9.9* |
24.8 |
2.50 |
5.68 |
| 2A |
2.9* |
6.1* |
2.08 |
4.65 |
| 4A |
4.3* |
10.2* |
2.36 |
5.36 |
| 7A |
15.3 |
29.7 |
1.94 |
6.42 |
| Mean |
2.22 |
5.53 |
||
|
Transforming growth factor beta 1 | ||||
|
Sample |
Green Signal |
Red Signal |
Array |
Real-Time |
| 1A |
8.8 |
11.5 |
1.30 |
2.43 |
| 2A |
10.3 |
18.2 |
1.77 |
1.64 |
| 4A |
14.9 |
12.8 |
-1.16 |
1.46 |
| 7A |
12 |
11.3 |
-1.06 |
6.91 |
| Mean | 0.21 | 3.11 | ||
Change in gene expression in tissue surrounding the surgical site compared to similar tissue from the non-surgically treated control eye based on real-time PCR (real-time) and microarrays (Array). Fold change values are arithmetic and not logarithmic. Also listed are the corresponding processed red and green signal values from the microarrays. The asterisk denotes signal values that are indistinguishable from background values and the double asterisk indicates signal values that are inordinately high.