Skip to main content
. Author manuscript; available in PMC: 2008 Sep 15.
Published in final edited form as: Antioxid Redox Signal. 2008 Aug;10(8):1463–1474. doi: 10.1089/ars.2007.2009

FIG. 6. CM-DCF fluorescence from single mature skeletal muscle fibers subjected to a period of electrically stimulated contractile activity with and without prior glutathione loading.

FIG. 6

(A). The relative fluorescence from control fibers (Inline graphic) compared with fibers that underwent contractile activity induced by electrical stimulation over the 15–30 min period (■). (B). Rate of change of relative DCF fluorescence in control fibers (□), fibers subjected to contractile activity induced by electrical stimulation over the 15–30 min period (■), and fibers pre -treated with 5 mM glutathione ethyl ester (GSHEE) for 2 h prior to CM-DCFH DA loading and subjected to contractile activity induced by electrical stimulation over the 15–30 min period (Inline graphic). *Statistically significant compared with control fibers during the same time period; #statistically significant compared with fibers subjected to contractile activity induced by electrical stimulation over 15–30 min period. Data were analyzed using a one-way ANOVA (p < 0.05), post hoc LSD (p < 0.05) and are shown as mean ± S.E.M, n 7–13 fibers in each group.