Figure 2.
Effects of recombinant light chain of clostridial NTs on SNARE proteins. Cellubrevin, TI-VAMP (A), SNAP25, SNAP23 (B), syntaxin 1a, and syntaxin 3 (C) were translated in vitro in the presence of [35S]methionine and then incubated with the various light chains of the NTs in 150 mM potassium glutamate, 10 mM HEPES-KOH, pH 7.2, for 12 h at 37°C. The cleavage was analyzed after the products were separated by SDS-PAGE and visualization of bands by autoradiography. The concentrations of the toxins used were as follows: TeNT, BoNT/B, D, F, and G, 2 μM; BoNT A, 0.8 μM; BoNT C, 0.9 μM; and BoNT E, 0.4 μM.