Table II.
Summary of characteristics of heIF5A mutant proteins.
Abbreviations, s.g.= slow growth. n.d. = not determined
| Mutant | Substrate for | Growth | Protein synthesis | |
|---|---|---|---|---|
| DHS | DOHH | |||
| Wild type | +++ | + | + | + |
| K47A | +++ | + | s.g. | n.d. |
| K47D | +++ | − | − | − |
| K47R | +++ | + | + | + |
| G49Aa | +++ | + | − | n.d. |
| K50A | − | − | − | n.d. |
| K50D | − | − | − | n.d. |
| K50I | − | − | − | n.d. |
| K50R | − | − | − | − |
| H51A | +++ | − | s.g. | n.d. |
| G52Aa | + | + | − | n.d. |
| K55A | + | + | − | n.d. |
| P74A | ++ | + | s.g. | n.d. |
| L91Ab | ++ | + | s.g. | n.d. |
| L101Ab | ++ | + | s.g. | n.d. |
a
In addition to the deoxyhypusine/hypusine at residue 50, these two glycine residues are likely the most critical residues in eIF5A/effector interactions.
b
These proteins appear to be unstable and their cellular levels were low.