Table 2.
Complete information and immunogen of primary antibodies applied
Antiserum | Species | Clonality | Working Dilution | Source Catalog/Lot Number | Immunogen |
---|---|---|---|---|---|
ChAT | Mouse | Monoclonal | 1:100 | Chemicon, Temecula, CA AB305/0704058097 | Choline acetyltransferase purified from rat brain |
GABA | Rabbit | Polyclonal | 1:1000 | Sigma-Aldrich, Saint Louis, MO A2052/106K4753 | GABA-BSA |
Glutamate | Rabbit | Polyclonal | 1:50 | Abcam, Cambridge, MA ab9440/180238 | L-glutamate conjugated to glutaraldehyde |
Enkephalin | Mouse | Monoclonal | 1:200 | Chemicon, Temecula, CA MAB350/0509010613 | Leu5-enkephalin conjugated to BSA |
c-Fos | Goat | Polyclonal | 1:100 | Santa Cruz Biotechnology, Santa Cruz, CA sc-52-G/G0706 | A synthetic peptide corresponding to the N-terminus residues 3−16 of human c-Fos molecule: FSGFNADYEASSSR |