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. Author manuscript; available in PMC: 2009 Aug 15.
Published in final edited form as: Life Sci. 2008 Jun 28;83(7-8):293–300. doi: 10.1016/j.lfs.2008.06.017

Figure 4.

Figure 4

A-B, Isobologram analyses of synergy between combinations of isoflavone and curcumin in BxPC-3 cells. The cells were incubated for 72 h in the absence (control) or presence of indicated concentrations of isoflavone and/or curcumin. Data points (*) are described by concentrations (in μM) of curcumin and isoflavone reflected on x- and y-axes respectively, and are representative of three independent experiments. Combination index (CI): a quantitative measure of the degree of drug interaction. CI < 1 indicates synergism; CI > 1 indicates antagonism; CI = 1 indicates additive effect.

C-D, Western blot analysis showing changes in the levels of Notch-1, Cyclin D1 and Bcl-xL as well as the levels of Bcl-2 in BxPC-3 and Colo-357 cells treated with isoflavone, curcumin, or their combination for 72 h. β-actin represents protein loading control.