Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Electrophoretic mobility shift assay (EMSA) showing the DNA binding activity of NF-κB in nuclear protein fractions from untreated (control), isoflavone-, curcumin-, or isoflavone + curcumin-treated BxPC-3 and Colo-357 cells. Super-shift assay showed that NF-κB band was shifted because of the formation of bigger complex after addition of anti- NF-κB p65 antibody. This assay confirmed the specificity of NF-κB binding to the DNA consensus sequence.
