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Two-step fluorescence screening of OBOC peptide library: A, DIC image of TentaGel beads; B, fluorescence positive beads. Bead 1, 3 are fluorescence transparent; bead 2 is fluorescence opaque; C, surface plot of fluorescence of beads in C; D, the fluorescence of fluorescence opaque beads disappeared after treatment with Gu.HCl; E, the fluorescence of fluorescence transparent beads remained after treatment with Gu.HCl; F, surface plot of fluorescence of beads in E.
