Table 3.
Composition of HPMA copolymer–peptide conjugates
| Conjugates | Structure | Mwa (kDa) | Pdb | Peptide (mol%)c | Peptide #/chain | Biotin (mol%)d | Biotin #/chain |
|---|---|---|---|---|---|---|---|
| PB | PHPMA-(PEG-biotin) | 63.2 | 1.7 | 0 | 0 | 0.4 | 1.8 |
| PB-A-B1 | PHPMA-(A-B1)-(PEG-biotin) | 42.1 | 1.4 | 2.1 | 5.2 | 0.34 | 1.0 |
| PB-A2-B1 | PHPMA-(A2-B1)-(PEG-biotin) | 39.0 | 1.3 | 2.0 | 4.7 | 0.23 | 0.6 |
| PB-A3-B1 | PHPMA-(A3-B1)-(PEG-biotin) | 38.7 | 1.3 | 2.2 | 4.9 | 0.45 | 1.2 |
| PB-A4-B1 | PHPMA-(A4-B1)-(PEG-biotin) | 41.1 | 1.3 | 1.7 | 4.2 | 0.34 | 1.0 |
| PB-A2-P1 | PHPMA-(A2-P1)-(PEG-biotin) | 55.6 | 1.5 | 1.1 | 4.3 | 0.36 | 1.4 |
| PB-A3-P1 | PHPMA-(A3-P1)-(PEG-biotin) | 37.3 | 1.5 | 1.83 | 3.7 | 0.31 | 0.8 |
PB HPMA copolymer backbone labeled with a PEG3400-biotin graft
a
Mw: weight-average molecular weight as determined with size exclusion chromatography
b
Pd: Polydispersity, ratio of weight-average molecular weight over number-average molecular weight
c
determined with amino acid analysis
d
determined with EZ Biotin Quantitation Kit