Table 4.
Crystallographic statistics for wild-type and mutant Acs.
| Wild-type AMP + | R194A | R584A | R584E | K609A CoA + | V386A CoA + | |
|---|---|---|---|---|---|---|
| Acetate + CoA | Propyl-AMP | Propyl-AMP | Propyl-AMP | PropylAMP | Propyl-AMP | |
| Resolution | 25 − 2.6 Å | 40 − 2.1 Å | 30 − 2.2 Å | 35 − 2.4 Å | 35 − 2.3 Å | 35 − 2.0 Å |
| Space Group | P212121 | P212121 | P212121 | P212121 | P212121 | P21 |
| Unit Cell | a= 90.9 Å | a= 91.7 Å | a= 91.8 Å | a= 92.0 Å | a= 91.6 Å | a= 59.9 Å |
| b= 94.9 Å | b= 95.5 Å | b= 95.4 Å | b= 95.5 Å | b= 94.5 Å | b= 143.0 Å | |
| c= 161.8 Å | c= 163.5 Å | c= 164.7 Å | c= 164.2 Å | c= 167.5 Å | c= 71.3 Å | |
| β= 90.9 | ||||||
| Rmerge a | 7.2% (22.4%) | 5.5% (27.9%) | 6.2% (31.4%) | 10.6% (36.4%) | 8.5% (39.9%) | 4.7% (21.3%) |
| Completenessa | 99.6% (100%) | 91.8% (58.4%) | 92.0% (61.6%) | 93.4% (81.0%) | 95.2% (81.0%) | 96.9% (85.1%) |
| I/σa | 17.5 (3.0) | 17.5 (2.4) | 20.1 (4.7) | 11.7 (2.0) | 11.8 (1.9) | 17.3 (4.2) |
| # Observations | 224405 | 259320 | 313104 | 173551 | 202699 | 200915 |
| # Reflections | 44408 | 77387 | 67992 | 55167 | 62317 | 59117 |
a
The number in parentheses represents the value for the highest resolution shell.