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. Author manuscript; available in PMC: 2009 Jul 15.
Published in final edited form as: Dev Biol. 2008 May 8;319(2):359–369. doi: 10.1016/j.ydbio.2008.04.035

Figure 9. Loss of Palisade results in defects in processing and cross-linking of sV17 and sV23, while loss of Cad99C has more limited effects on cross-linking.

Figure 9

Equal numbers of Stage 14 egg chambers (14) or laid eggs (LE) were loaded in each lane, following extraction by boiling in Laemmli sample buffer with (+) or without (-) 100 mM DTT as indicated above the top row of panels. Blotting was with either anti-sV17 (top panels) or anti-sV23 (bottom panels), and positions of the major Stage 14 processed forms of sV17, sV23, and a cross-reacting material (CRM) are indicated at right. In wild-type (OR), sV17 and sV23 are only soluble in the Stage 14 egg chambers extracted with DTT, while psd null and Cad99C null show solubility of a ladder of partially cross-linked forms of these proteins in laid eggs. The psd null also shows persistence of incompletely processed forms in Stage 14 egg chambers (marked with asterisks), which together with a small amount of correctly processed sV23 fail to be disulfide cross-linked during oogenesis.