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Crosslinking of constructs lacking either TM1 or TM2. CT constructs lacking TM2 and containing single cysteine substitutions at the indicated positions in TM1, and NT constructs lacking TM1 and containing single cysteine substitutions in TM2, were crosslinked for the indicated times as described for full length CD39 in Figure 4. Identical results were obtained in the presence of 5 mM ATP or in the presence of 0.005% Triton X-100 or 20 μM OA.
