Table 1.
Steady State Rate Constants of Unnatural Terminus Extension by Insertion of dCTPa
| 5′ - dTAATACGACTCACTATAGGGAGA (X) | ||||
|---|---|---|---|---|
| 3′ - dATTATGCTGAGTGATATCCCTCT (Y) GCTAGGTTACGGCAGGATCGC
| ||||
| X | Y | kcat (min−1) | KM (μM) | kcat/KM(M−1min−1) |
| BFr | 4MP | 5.4 ± 1.2 | 6.1 ± 2.1 | 8.8 × 105 |
| BTp | 4MP | 3.3 ± 1.0 | 11.5 ± 1.3 | 2.9 × 105 |
| BTz | 4MP | 5.4 ± 1.7 | 7.6 ± 2.2 | 7.1 × 105 |
| IN | 4MP | 5.3 ± 1.2 | 3.9 ± 0.9 | 1.4 × 106 |
| 4MP | BFr | 30.9 ± 6.5 | 1 | 1.4 × 105 |
| 4MP | BTp | 19.8 ± 5.1 | 205 ± 39 | 9.7 × 104 |
| 4MP | BTz | 14.9 ± 3.3 | 89 ± 23 | 1.7 × 105 |
| 4MP | IN | 15.8 ± 4.1 | 402 ± 108 | 3.9 × 104 |
a
See supporting information for experimental details.