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. Author manuscript; available in PMC: 2008 Sep 15.
Published in final edited form as: J Am Chem Soc. 2007 Aug 8;129(34):10466–10473. doi: 10.1021/ja072276d

Figure 2.

Figure 2

(A) Sequence of 18-nt primer and 29-nt template used for full length synthesis. (B) Full length synthesis by KF exo−; 40 nM primer-template, 1.23 nM enzyme, 50 mM Tris-HCl, pH 7.5, 1 mM DTT, 50 μg/mL acetylated BSA, 2 hours at 25 °C, 5 mM MgCl2, 1 mM MnCl2, and 50 μM of each dCTP and dGTP, with (lane 1) or without (lane 2) 200 μM d3FBTP. (C) Full length synthesis by KF exo+; 40 nM primer-template, 1.23 nM enzyme, 50 mM Tris-HCl, pH 7.5, 1 mM DTT, 50 μg/mL acetylated BSA, 2 hours at 25 °C, 10 mM MgCl2, 1 mM MnCl2, and 200 μM dCTP/dGTP, with (lane 1) or without (lane 2) 200 μM d3FBTP.