Figure 8.

Induction and subcellular fractionation of Pex19p. (A) Glucose-grown wild-type cells (PPY12) were shifted to oleate and methanol media, and samples were collected at the indicated times and processed as described for Figure 3 for immunoblotting with anti-Pex19p antibodies. (B) Oleate-grown spheroplasts of wild-type and pex19Δ cells (SMD1163 and SKF14) were lysed and subjected to sequential differential centrifugation. Equivalent amounts of the PNS, 27,000 × g supernatant (S27), 27,000 × g pellet (P27), 100,000 × g supernatant (S100), and 100,000 × g pellet (P100) were resolved by SDS-PAGE, transferred to nitrocellulose, and probed with anti-Pex19p antibodies.