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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Cancer Res. 2008 Jul 1;68(13):5023–5030. doi: 10.1158/0008-5472.CAN-07-6189

Figure 1.

Figure 1

Structural and functional demonstration of BRCA2 gene disruption. A, targeting scheme. OF and OR, primers outside the deleted sequence; IF and IR, primers inside the deleted sequence; SF, SR, SF2, and SR2, screening primer pairs; LHA and RHA, left and right homology arms. Numbers on light background indicate exons, and numbers on dark background indicate BRC repeats. B, PCR detecting the wt and targeted alleles using genomic DNA as template. C, Northern blot detecting the targeted exon 11 mRNA transcript.