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. 2008 Sep 10;4(5):309–317. doi: 10.7150/ijbs.4.309

Figure 2.

Figure 2

PCR analyses of (A) 6-phosphogluconate dehydrogenase (EC: 1.1.1.44) and (B) O-acetyl homoserine (thiol) lyase (EC: 2.5.1.49). Panel A. PCR products amplified from genomic DNA of P. aeruginosa PAO1, P. putida KT2440 and P. syringae pv. tomato DC3000 using ppu44 primers (Lanes 1-4) and pst44 primers (Lanes 5-8). The 215-bp PCR products of the ppu44 gene fragment were observed in lane 1 (P. aeruginosa), lane 2 (P. putida) and lane 3 (P. syringae pv. tomato). The 525-bp pst44 amplified fragments were seen in lane 5 (P. aeruginosa) and lane 7 (P. syringae pv. tomato). No bands were observed in lane 6 (P. putida), lanes 4 and 8 (negative control). Panel B. PCR products amplified from genomic DNA of P. aeruginosa PAO1, P. putida KT2440 and P. syringae pv. tomato DC3000 using pae49 primers (Lanes 1-4) and ppu49 primers (Lanes 5-8). The 403-bp PCR products of the pae49 gene fragment were observed in lane 1 (P. aeruginosa) and lane 3 (P. syringae pv. tomato). No bands were seen in lane 2 (P. putida) and lane 4 (negative control). The 406-bp ppu49 gene fragment was seen in lane 6 (P. putida). No bands were observed in lane 5 (P. aeruginosa), lane 7 (P. syringae pv. tomato) and lane 8 (negative control).