Table 4.
Results of the PCR and dot-blot hybridization analyses. (+/-) showing the presence or absence of enzymes. pae- Pseudomonas aeruginosa chromosomal DNA, ppu- Pseudomonas putida chromosomal DNA and pst- Pseudomonas syringae pv. tomato chromosomal DNA. NA- Information Not Available.
| Enzymes | Primer/Probe | pae | ppu | pst |
|---|---|---|---|---|
| 6-phosphogluconate dehydrogenase | KEGG database | - | + | + |
| (EC: 1.1.1.44) | SYSTOMONAS | + | + | + |
| PGD | - | + | NA | |
| ppu44 primers | + | + | + | |
| pst44 primers | + | - | + | |
| ppu44 probe | + | + | + | |
| Comment | Probably + | + | + | |
| Xylulose kinase | KEGG database | + | - | + |
| (EC: 2.7.1.17) | SYSTOMONAS | + | NA | + |
| PGD | + | NA | NA | |
| pae17 primers | + | - | + | |
| pst17 primers | - | - | + | |
| pst17 probe | + | - | + | |
| + | - | + | ||
| O-acetyl homoserine (thiol) lyase | KEGG database | + | + | - |
| (EC: 2.5.1.49) | SYSTOMONAS | + | + | NA |
| PGD | NA | NA | NA | |
| pae49 primers | + | - | + | |
| ppu49 primers | - | + | - | |
| pae49 probe | + | + | + | |
| Comment | + | + | Probably + |