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. Author manuscript; available in PMC: 2008 Sep 16.
Published in final edited form as: Biochemistry. 2006 Mar 28;45(12):3863–3874. doi: 10.1021/bi052252o

Figure 8.

Figure 8

Type V collagen from fraction V2 is an MMP-20 substrate. (A) chromatogram of purified fraction V2 demonstrating a high level of V2 purity. Note that arrows point to a background trace from the chromatogram in Figure 6 demonstrating that the peak shown was from the original V2 fraction. (B) chromatogram of fraction V2 after 20 h digestion with rhMMP-20. Note that the peak at approximately 45 min was substantially diminished after the digestion. (C) SDS-PAGE gel (left) and Western blot (right) demonstrating that fraction V2 Type V collagen was digested after 20 h exposure to rhMMP-20 (lane 20) as compared to the 0 h digestion (lane 0) and the Type V collagen without rhMMP-20 (lane C).