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. 2008 Jul 29;295(3):E692–E697. doi: 10.1152/ajpendo.90370.2008

Fig. 4.

Fig. 4.

Left: representative Western blots of the IRS-associated p85 kDa regulatory subunit of phosphoinositide 3-kinase (PI3K; determined after immunoprecipitation with IRS-1 or IRS-2 antibodies). Bar graphs show means ± SE of IRS-1- or IRS-2-associated PI3K (p85; n = 6) pre- and 4 h post-saline or euglycemic hyperinsulinemic clamping without (post-ins) and with lipid-heparin (post-ins+lip). Right: representative Western blots of phospho-ERK1 and phospho-ERK2 and unphosphorylated ERK1 (p44) and ERK2 (p42). Bar graphs show means ± SE (n = 4) of phospho-ERK1/ERK1 ratios (top) and phospho-ERK2/ERK2 (bottom).